If the collection cup has a lid, remove it carefully and set it down with the inner surface up. Do not touch the inside of the cup with your fingers.
Clean the area around your genitals.
A man should retract the foreskin, if present, and clean the head of his penis with medicated towelettes or swabs.
A woman should spread open the genital folds of skin with one hand. Then use her other hand to clean the area around the urethra with medicated towelettes or swabs. She should wipe the area from front to back so bacteria from the anus is not wiped across the urethra.
Begin urinating into the toilet or urinal. A woman should hold apart the genital folds of skin while she urinates.
After the urine has flowed for several seconds, place the collection cup into the urine stream and collect about 2 fl oz (60 mL) of this "midstream" urine without stopping your flow of urine.
Do not touch the rim of the cup to your genital area. Do not get toilet paper, pubic hair, stool (feces), menstrual blood, or anything else in the urine sample.
Finish urinating into the toilet or urinal.
Carefully replace and tighten the lid on the cup then return it to the lab. If you are collecting the urine at home and cannot get it to the lab in an hour, refrigerate it.
Urine collection over 24 hours
You start collecting your urine in the morning. When you first get up, empty your bladder but do not save this urine. Write down the time that you urinated to mark the beginning of your 24-hour collection period.
For the next 24 hours, collect all your urine. Your doctor or lab will usually provide you with a large container that holds about 1 gal (4 L). The container has a small amount of preservative in it. Urinate into a small, clean container and then pour the urine into the large container. Do not touch the inside of either container with your fingers.
Keep the large container in the refrigerator for the 24 hours.
Empty your bladder for the final time at or just before the end of the 24-hour period. Add this urine to the large container and record the time.
Do not get toilet paper, pubic hair, stool (feces), menstrual blood, or other foreign matter in the urine sample.
How It Feels
There is no discomfort in collecting a one-time or 24-hour urine sample.