If the collection cup has a lid, remove it carefully. Set it down with the inner surface up. Do not touch the inside of the cup with your fingers.
Clean the area around your genitals.
For men: Pull back the foreskin, if you have one. Clean the head of the penis thoroughly with medicated towelettes or swabs.
For women: Spread open the folds of skin around the vagina with one hand. Then use your other hand to clean the area around the vagina and urethra thoroughly with medicated towelettes or swabs. Wipe the area from front to back to avoid spreading bacteria from the anus to the urethra.
Start urinating into the toilet or urinal. Women should keep holding apart the folds of skin around the vagina while they urinate.
After the urine has flowed for several seconds, place the collection cup into the stream. Collect about 3 fl oz (90 mL) of this "midstream" urine without stopping the flow.
Do not touch the rim of the cup to your genital area. And don't get toilet paper, pubic hair, stool (feces), menstrual blood, or other foreign matter in the urine sample.
Finish urinating into the toilet or urinal.
Carefully replace the lid on the cup. Return the cup to the lab. If you are collecting the urine at home and can't get it to the lab in an hour, refrigerate it.